Active substances: Ciprofloxacin
Compare the time to discharge, time to discontinuation of any antimicrobial therapy, and time to defervescence of patients treated with these regimens.
Compare 28-day survival of patients treated with these regimens. Determine the proportion of these patients who are eligible for oral therapy and a therapeutic management including intention of early discharge.
Determine the medical and nonmedical reasons for continued in-hospital observation and care or for readmission of these patients.
For each antibiotic, in the upper part of the panel the OD of the STX-specific signal is plotted against the dilution of the supernatants. In the lower part of each panel, the STX-titers are shown which were determined in the plots of the OD as indicated exemplarily for the 1 x green dashed lines and 4 x red dashed lines MIC of ciprofloxacin.
Briefly, from the OD-value of the undiluted sample of the untreated culture a horizontal dashed line was drawn until it intersected the plot of a given MIC.
From this intersection a vertical line was drawn to determine the dilution at which the OD-value of the respective supe rnatant equaled the OD-value of the untreated control.
The inverse of this dilution was defined as the STX-titer of the sample. Full size image Fosfomycin at subinhibitory concentrations as well as at the 4 x MIC increased the titers of STX of supernatants of strain O 157: H 7 up to 4-fold as compared to untreated controls, while fosfomycin did not significantly affect titers of STX 2 in cultures of O 104: H 4 Figure 2 C.
Fosfomycin has already been discussed as a risk factor increasing clinical symptoms in an outbreak of STEC O 157: H 7 among school children.
Rifampicin at gradually increasing concentrations in the range of 0. Both STX 1 and 2 inhibit protein synthesis in eukaryotic cells and thereby kill Vero cells.
The cell free supernatants of STEC cultures described in Figure 2 were 10-fold serially diluted and added to semi-confluent monolayers of Vero cells in microtiter plates.
After incubation for 24 h, XTT-labeling reagent was added and cultures were incubated for another 24 h before measuring the viability of the Vero cells as OD 450 of the samples.